The protein concentrations of supernatants after centrifugation (twelve,000 g, five min, 4uC) have been calculated with a Pierce BCA assay package (Thermo Scientific, Scoresby, Victoria, Australia) in accordance to manufacturer’s guidance
Unaffected South Hampshire and Merino sheep, and CLN5 (Borderdale) heterozygous (CLN5+/2, unaffected) animals ...