ine width in the edge indicates the strength of the information support. (B) The top

ine width in the edge indicates the strength of the information support. (B) The top 20 genes on the PPI network: X-axis represents degree, and Y-axis indicates genes.FIGURE 5 | Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of targets. (A) GO evaluation of targets: X-axis represents GO terms, and Y-axis indicates the enrichment score [adjusted false discovery rate (FDR)] in that term. (B) KEGG analysis of targets: Dot plot showed the major 20 KEGG pathways with adjusted FDR. The color scale indicates the adjusted FDR, along with the dot size represents the gene count in every term.= 86, HQ15), baicalein (degree = 83, CQC2), stigmasterol (degree = 76, M), isorhamnetin (degree = 72, HQ5), dinatin (degree = 71, CQC1), jaranol (degree = 62, HQ2), 6-OH-luteolin(degree = 62, CQC5), and scutellarein (degree = 59, CQC9). Ultimately, we chose quercetin, luteolin, BD2 custom synthesis kaempferol, scutellarein, and stigmasterol to treat pressure diarrhea in mice.Frontiers in Veterinary Science | frontiersin.orgOctober 2021 | Volume 8 | ArticleZhang et al.Anti-diarrhea Mechanism Evaluation of QJCTABLE 2 | Establishment of a mouse model of strain diarrhea. Group Standard control group Model manage groupa,b SignificantThe accumulated variety of stools 3.eight 0.838a 11.6 1.141bThe quantity of loose stools 0a 7.8 0.837bThe rates of loose stools ( ) 0a 0.68 0.074bdifferences at p 0.05.FIGURE 6 | Therapy of diarrhea mice with gradient dose of QJC. Data were expressed as the mean SD (n = five); the important distinction involving groups (p 0.05) was shown by the distinctive letters above the histogram. (A) The accumulated quantity of stools. (B) The time of initial diarrhea. (C) The amount of loose stools. (D) The prices of loose stools.Protein rotein Interaction Network AnalysisThe PPI network comprised 203 nodes and 595 edges (Figure 4A), which indicated 595 interactions. To clarify this further, the prime 20 nodes had been extracted by the degree (Figure 4B). Inside the interaction network, amyloid beta A4 protein (APP; degree = 34), phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1; degree = 27), Src protein-tyrosine kinase (SRC; degree = 26), B2 bradykinin receptor (B2R; degree = 24), and IL-8 (degree = 24) will be the major 5 potential targets of QJC within the therapy of diarrhea, which may play a crucial part.involved in 44 enrichment results, along with the HD2 review cellular element (CC) was involved in 73 enrichment results. Based on their enrichment score, we visualized the top rated ten pathways (Figure 5A). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was employed to discover the potential mechanisms of QJC within the remedy of tension diarrhea. Consequently, 130 KEGG pathways have been obtained, and also the top rated 20 KEGG signaling pathways were constructed depending on FDR (Figure 5B).High-Performance Liquid Chromatographic ResultsQuercetin, kaempferol, luteolin, scutellarein, and stigmasterol have been eluted, as shown in Supplementary Figure 1. Meanwhile, we calculated the content material of different elements in the sample applying the regression equation. Quercetin, kaempferol, luteolin, scutellarein, and stigmasterol levels were 4.91 0.228, 2.18 0.237, 25.58 0.543, 0.53 0.026, and 145.72 13.712 /g, respectively (Supplementary Table 2).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes Enrichment AnalysesTo additional explore the biological characteristics from the 297 candidate targets of QJC, we performed GO analysis on diarrhea targets. Consequently, the biological course of action (BP) was involved in 292 enr