results, obtained by product ion scan mode analysis, had been observed from the product ion

results, obtained by product ion scan mode analysis, had been observed from the product ion spectra obtained right after the isolation of m/z 227.0 on Q1. This precursor ion could be the product ion spectra obtained right after the isolation of m/z 227.0 on Q1. This precursor ion is most likely represent the molecular ion ofionallegedH1 Receptor Agonist MedChemExpress alleged metabolite of 5, by de-nitration of probably to to represent the molecular an of an metabolite of five, obtained obtained by denitration of your side chain. Figure 9a reports the comparison of your m/z 227.0 chromatographic profiles obtained in the rat liver microsomal GlyT2 Inhibitor Formulation fraction just before the incubation with compound five (dotted line) and immediately after two hours’ incubation (continuous line). A chromatographic peak is evident at the retention time of two.60 min only in theAntioxidants 2022, 11,12 ofthe side chain. Figure 9A reports the comparison on the m/z 227.0 chromatographic profiles obtained in the rat liver microsomal fraction ahead of the incubation with compound five (dotted line) and immediately after two hours’ incubation (continuous line). A chromatographic peak is evident at the retention time of two.60 min only within the second profile, viz. after two hours’ incubation. The corresponding solution ion spectrum, depicted in Figure 9B, exhibits the Antioxidants 2022, ten, x FOR PEER Evaluation 13 of 21 loss of consecutive fragments in the side chain and it is actually compatible with the supposed metabolite’s structure.Figure 9. (a) Superimposed mass chromatograms of thethe m/z 227.0 precursor ion, obtained from Figure 9. (A) Superimposed mass chromatograms of m/z 227.0 precursor ion, obtained from the rat liverliver microsomal fractiont at t0=(dotted line) and t = = two h (continuous line)incubation together with the rat microsomal fraction at = 0 (dotted line) and t two h (continuous line) incubation with compound 5. (b) Product ion spectrum in the selected m/z 227.0 precursor, collected at two.60 min, compound five. (B) Solution ion spectrum on the chosen m/z 227.0 precursor, collected at 2.60 min, in the latter evaluation. in the latter analysis.Analogue experiments were executed around the rat liver microsomal fraction incubated Analogue experiments have been executed on the rat liver microsomal fraction incubated with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds to the molecular with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds to the molecularalleged metabolite 7 metabolite 7 obtained right after single the side chain.with the side ion of the ion in the alleged obtained following single de-nitration of de-nitration Figure 10A chain. Figure 10a reports the m/z 288.0 chromatographic profiles obtained from the rat reports the comparison of your comparison with the m/z 288.0 chromatographic profiles obtained from the fraction prior to the incubationbefore compound 7 and after two hours, liver microsomal rat liver microsomal fraction using the incubation with compound 7 and soon after two This time, a chromatographic peak is evident in the retention time of 3.78 respectively. hours, respectively. This time, a chromatographic peak is evident at the retention time of 3.78 min only rat the profile from the rat liver microsomal fraction min only in the profile from the in liver microsomal fraction collected immediately after two hours’ collected just after two hours’ incubation. The ion spectrum, depicted ion Figure 10B, exhibits incubation. The corresponding product corresponding item in spectrum, depicted in Figure 10b, exhibits a fragmentation related to Figure 9b. The solution ion spe