ACE2 in enterocytes), SLC7A9 (which codes for an L-DOPA influx transporter) and SLC16A10 (which codes

ACE2 in enterocytes), SLC7A9 (which codes for an L-DOPA influx transporter) and SLC16A10 (which codes for an L-DOPA efflux transporter). In the whole set of information (n = six, two manage samples, two samples at 24 h post-infection and two samples at 60 h post infection), we could extract expression values for 11 out of 14 genes of interest. We then made use of the Pearson’s correlation test to evaluate the co-expression hyperlinks in between these genes and ACE2. We found that eight important genes involved within the metabolism of dopamine and/or trace amines exhibited statistically substantial co-expression hyperlinks with ACE2 across all experimental situations. Of note, by far the most robust correlation link was observed for MAOB, followed by SLC7A9 and SULT1A1 (Table 3).Int. J. Mol. Sci. 2021, 22,tern. Furthermore anticipated, the L-DOPA efflux transporters SLC3A2 and SLC7A8 had been detected at the basolateral membrane of enterocytes. A low and diffuse staining pattern was observed for SLC16A10. Lastly, no TH staining could be detected (Figure S1), in accordance with genomics analyses. Based on these mined information, a scheme summarizing the predicted dopamine/trace amines metabolic pathways taking spot in human enterocytes six of 16 is shown in Figure 2.Figure 2. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking place in human Figure 2. Functional scheme summarizing the predicted dopamine/trace amines metabolic pathways taking place in huenterocytes of of smaller JNK1 medchemexpress intestine. This scheme is depending on the mining of human expression atlases and on previously man enterocytesthe the smaller intestine. This scheme is based onthe mining of human expression atlases and on previously publishedbiochemical and/or functional information obtained in intestinal or non-intestinal cells. The molecules integrated within this published biochemical and/or functional data obtained in intestinal or non-intestinal cells. The molecules included within this scheme comprise: angiotensin-converting enzyme (ACE2), solute carrier family members six member 19 (SLC6A19), solute carrier scheme comprise: angiotensin-converting enzyme two 2 (ACE2), solute carrier family six member 19 (SLC6A19), solute carrier family members 33member 11(SLC3A1), solute carrier family 77member 99(SLC7A9), dopa-decarboxylase (DDC), sulfotransferase household member (SLC3A1), solute carrier family member (SLC7A9), dopa-decarboxylase (DDC), sulfotransferase family members 1A member 11 (SULT1A1),sulfotransferase family 1A member 22 (SULT1A2),sulfotransferase family 1A member 33 household 1A member (SULT1A1), sulfotransferase loved ones 1A member (SULT1A2), sulfotransferase family 1A member (SULT1A3), cytochrome P450 loved ones two subfamily D member 6 (CYP2D6), monoamine IRAK4 Storage & Stability oxidase A (MAOA), monoamine oxidase B (MAOB), solute carrier loved ones 3 member two (SLC3A2), solute carrier household 7 member 8 (SLC7A8) and solute carrier family 6 member ten (SLC16A10). Table 3. Correlation analysis of ACE2 mRNA levels with crucial genes of the dopamine/trace amines metabolic pathways in SARS-CoV2-infected human enterocytes. DDC 0.84 0.035 MAOA 0.86 0.025 MAOB 0.96 0.001 SULT1A1 0.92 0.007 SLC7A9 0.95 0.003 SLC3A1 0.87 0.02 SLC6A19 0.88 0.017 SLC3A2 0.9 0.Expression information have been extracted from Lamers et al. [34] along with the Pearson’s test was applied to assess correlation coefficient (r, upper line) and statistical significance (p-value, decrease line)) involving ACE2 and genes of interest. Gene symbols: dopa-decarboxylase (DDC), monoamine oxidase A (MAOA), monoamine oxidase B (MAOB), solute carr