0.0 00 0.0 0.090 90 090 90 0 00 0020 20 2040 40 4060 60

0.0 00 0.0 0.090 90 090 90 0 00 0020 20 2040 40 4060 60 6080 80 80100 100 10020 20 2040 40 4060 60 6080 80 80100 one hundred 100Post-activation time, s s Post-activation time, Post-activation time, s s Post-activation
0.0 00 0.0 0.090 90 090 90 0 00 0020 20 2040 40 4060 60 6080 80 80100 one hundred 10020 20 2040 40 4060 60 6080 80 80100 one hundred 100Post-activation time, s s Post-activation time, Post-activation time, s s Post-activation time, (a)Post-activation time, s s Post-activation time, Post-activation(b) s s time, Post-activation time,CP 2424 CP CP 4848 CP PLGA 200 24h PLGA 200 24h CP 2424 200 48h CP PLGA 200 48h PLGA CP 4848 200 72h CP PLGA 200 72h PLGA PLGA 200 24h PLGA 24h PLGA 3535 24h PLGA 200 24h PLGA 200 48h PLGA 48h PLGA 3535 48h PLGA 200 48h PLGA 200 72h PLGA 72h PLGA 3535 72h PLGA 200 72h PLGA 3535 24h PLGA 24h PLGA 3535 48h PLGA 48h PLGA 3535 72h PLGA 72hFigure 7. Kinematic parameters of of sterlet spermatozoa obtained following hormone treatments. (a)dynamics post-activa(a) (b) Figure 7. Kinematic parameters sterlet spermatozoa obtained soon after hormone therapies. (a) VCL VCL dynamics posttion. (b) LIN dynamics post-activation. Data are Mouse web presented as meanmean (dots)quadric polynomial regression lines.lines. activation. (b) LIN dynamics post-activation. Data are presented as (dots) and and quadric polynomial regression AbFigure 7. Kinematic parameters of sterlet spermatozoa obtained following hormone remedies. collected at 24 and 48 h postbreviations: CP 24 h, CP 48 h-treatment by carp pituitary extract, dose four mg/kg, samples (a) VCL dynamics post-activaAbbreviations: CP 24 h, CP 48 h-treatment by carp pituitary mean (dots) andmg/kg, samples collected at 24 lines.48 h tion. (b) LIN dynamics post-activation. Data are presented as extract, dose 4 quadric polynomial regression and Abpost-injectionCP 24 h, CP 48 h-treatment by carp200 48 h, PLGA 200dose 4 mg/kg, samples collected at 24 /kg, samples breviations: respectively; PLGA 200 24 h, PLGA pituitary extract, 72 h–treatment by Alarelin, dose 200 and 48 h postcollected at 24, 48, and 72 h post-injection respectively; PLGA 35 24 h, PLGA 35 48 h, PLGA 35 72 h–treatment by Alarelin, dose 35 /kg, samples collected at 24, 48, and 72 h post-injection respectively.Animals 2021, 11, x10 Fmoc-Gly-Gly-OH Formula ofinjection respectively; PLGA 200 24 h, PLGA 200 48 h, PLGA 200 72 h–treatment by Alarelin, dose 200 g/kg, samples collected at 24, 48, and 72 h post-injection respectively; PLGA 35 24 h, PLGA 35 48 h, PLGA 35 72 h–treatment by Alarelin, Animals 2021, 11, 3305 ten of 13 dose 35 g/kg, samples collected at 24, 48, and 72 h post-injection respectively.Spermatozoon Motility RateSpermatozoon Motility Price Two-way ANOVA applied to motility rate revealed a substantial effect of therapy (p 0.001). In contrast, effects of time post-injection as well as the interaction of those two aspects Two-way ANOVA applied to motility rate revealed a significant effect of treatment were not important (p = 0.989 and p = 0.751, respectively). Tukey’s test these two things (p 0.001). In contrast, effects of time post-injection and the interaction ofwas utilised to examine imply values of = 0.989 and p = 0.751, respectively). Tukey’s groups without considwere not significant (pspermatozoon motility rate in experimentaltest was used to evaluate eration of time spermatozoon motility price in experimental groups devoid of consideration mean values of post-injection. Motility rate was significantly reduced in the PLGA200 group than in PLGA35, whilst no differences had been found in between in along with the PLGA groups (Figof time post-injection. Motility price was considerably reduce CPthe PLGA200 group than in ure eight). PLGA35, whilst no variations were identified between CP as well as the PLG.