Liquid culture).Table 1. C. aquatica samples grown beneath distinct situations. Sample Code A3 A4 A6

Liquid culture).Table 1. C. aquatica samples grown beneath distinct situations. Sample Code A3 A4 A6 A7 A9 B1 B3 B5 D1 D3 D4 E2 E3 E4 Culture liquid liquid liquid liquid liquid liquid liquid liquid liquid liquid liquid strong solid strong Medium wheat straw wheat straw wheat straw wheat straw wheat straw alder leaves alder leaves alder leaves malt extract malt extract malt extract wheat straw wheat straw wheat straw Growth Phase exponential exponential stationary stationary stationary exponential exponential exponential exponential exponential exponential NA NA NA Variety of Reads 50,666,214 57,490,827 16,839,738 17,367,382 17,584,532 17,922,153 16,700,439 14,857,484 17,796,408 17,863,281 19,469,408 16,859,013 16,904,004 18,861, Growth phases seem simultaneously in solid culture and were not separated.Gene expression showed strong variations among strong and liquid cultures, using the most differentially expressed genes getting amongst development in strong culture compared to stationary growth in liquid culture plus the second-most differentially expressed genes among strong growth and exponential growth in liquid culture. The distinction amongst stationary and exponential growth was weaker by comparison (see Table two). Development on both plant matter substrates induced related modifications within the metabolism in comparison with malt extract, indicated by a considerable (Fisher’s precise test, p 1092) overlap between differentially expressed genes.Table 2. Quantity of up- and down-regulated genes for distinctive comparisons. Comparison Condition 1 exponential growth on wheat straw in liquid culture exponential development on alder leaves in liquid culture exponential development on wheat straw in liquid culture development on wheat straw in solid culture development on wheat straw in solid culture Condition two exponential growth on malt extract in liquid culture exponential growth on malt extract in liquid culture stationary development on wheat straw in liquid culture exponential growth on wheat straw in liquid culture stationary development on wheat straw in liquid culture Differential Expression Upregulated 1430 1033 1380 2731 2683 Downregulated 1570 1462 883 2328J. Fungi 2021, 7, 854 J. Fungi 2021, 7, x FOR PEER REVIEW8 of 14 eight ofThe 5 recognized laccase genes, too as the eight newly identified laccase-like genes, The five recognized laccase genes, at the same time as the eight newly identified laccase-like genes, showed no constant FG9065 MedChemExpress pattern of up- or down-regulation Compound E site inside the exponential development phase showed no consistent pattern of up- or down-regulation within the exponential development phase in liquid culture when comparing alder and wheat straw (Figure 1). In the six identified in liquid culture when comparing alder and wheat straw (Figure 1). On the six identified putative Class IIII peroxidases, two had been upregulated on wheat straw and one particular was downputative Class peroxidases, two had been upregulated on wheat straw and one particular was downregulated on wheat straw. For development on alder, no important differential expression was regulated on wheat straw. For development on alder, no important differential expression was observed for the putative peroxidases (Figure 1). Of the 137 achievable cytochrome P450 observed for the putative peroxidases (Figure 1). With the 137 probable cytochrome P450 proteins, 33 have been up- and 18 down-regulated in wheat straw, and 20 were up- and 24 proteins, 33 had been up- and 18 down-regulated in wheat straw, and 20 had been up- and 24 down-regulated on alder (Figure 1). down-regulated on alder (Figure 1).Figure.