Nterests.Authors’ ContributionsJun Han, Hang-Hang Xu, Xiao-Long Chen, Hao-Ran Hu, and Kun-Mei Hu Performed the experiments and analyze information. Jun Han and Guo-Wei He wrote up the manuscript. Jun Han and Zhi-Wu Chen made the protocol and discussion around the benefits. Guo-Wei He contributed to critical discussion on the study design and style, benefits, presentation, writing up, and submission.AcknowledgmentsThe authors appreciated the contributions in the other members of our laboratory: ZL QIN, H YU, FY FAN, and XW LI. This study was supported by the National Natural Science Foundation of China under Grant no. 81173596; the All-natural Science Foundation of your Department of Education of Anhui province beneath Grant no. Reactive Blue 4 Purity & Documentation KJ2015A157. This study was supported by an unrestricted grant from Recordati SpA.AbbreviationACh: BCA: CIR: CBA: EDHF: EEG: GAPDH: IR: PGI2 : NO: PSS: RR: SD rats: TRP: TRPV: TFR: Acetylcholine Bicinchoninic acid Cerebral ischemia-reperfusion Cerebral basal artery Endothelium-dependent hyperpolarizing factor Electroencephalograph Glyceraldehyde 3-phosphate dehydrogenase Ischemia/reperfusion injury Prostacyclin Nitric oxide Precooled physiological salt option Ruthenium red Sprague-Dawley rats Transient receptor prospective TRP vanilloid channel Total flavonoids of Rhododendron.
Rheumatoid arthritis (RA) is actually a chronic, systemic, and autoimmune illness. It may result in irreversible joint destruction and 89-74-7 References deformity, seriously affecting people’s good quality of life [1]. Disease-modifying antirheumatic drugs, anti-inflammatory drugs, and painkillers have long been used to treat RA [2]. Using the improvement of medicine, more and more attention is paid to diverse therapeutic strategies, as well as a quantity of individuals pick adjuvant therapies and complementary and alternative medicine to treat their RA. As a critical element of complementary and alternative medicine, Zhuang medicine plays an essential part in treating RA. Longzuan Tongbi Formula (LZTB) is an successful verified prescription in Zhuang medicine for treating active RA. It consists of Toddalia asiatica (TA), Kadsura coccinea (KC), Alangium chinense (AC), Sinomenium acutum (SA), Bauhinia championii (BC), Spatholobus suberectus (SS), Zanthoxylum nitidum (ZN), and Ficus hirta Vahl (FHV). Clinical research have shown that LZTB can considerably lessen RA patients’ erythrocyte sedimentation rate, and that it has a very good therapeutic effect on enhancing RA patients’ morning stiffnessand joint discomfort [3]. Nevertheless, its active components, underlying targets, and pharmacological mechanism in treating RA are still not clear. Compound preparations in conventional medicine can act on many targets via multicomponent reaction, play an indispensable function in core pathways in illnesses, and enable realize the purpose of treating illnesses [4]. Much consideration has been provided to the study in the multicomponent and multitarget action mechanism. With all the rapid improvement of bioinformatics, network pharmacology has become a new approach to correctly and systematically study the mechanism of action, security, along with other elements of compound preparations in traditional medicine [5]. For instance, Tang et al. have applied network pharmacology to study the mechanism of action of XuanHuSuo Powder in treating osteoarthritis [8], Liu et al. have adopted a network pharmacology approach in exploring the pharmacological mechanism of Xiaoyao Powder on anovulatory infertility [9], and Li et al. have analyzed the therapeutic effects of Zi Dian.
