Ect acting mutagens, 2-aminoanthracene (2-AA) and benzo[a]pyrene (B(a)P) were performed with S. typhimurium TA102 and

Ect acting mutagens, 2-aminoanthracene (2-AA) and benzo[a]pyrene (B(a)P) were performed with S. typhimurium TA102 and TA98 assay systems. In addition, the enzymatic and nonenzymatic methods were employed to evaluate the anti-oxidative effects of the tested extracts. Results: A significant buy Tirabrutinib effect against the Gram positive and Gram negative reference bacterial strains was observed with all the extracts. The mutagenic and antimutagenic studies revealed that all the extracts decreased the mutagenicity induced by B(a)P (7.5 g/plate), 2-AA (5 g/plate), MMS (1.3 mg/plate) and NOPD (10 g/plate). Likewise, all the extracts showed an important free radical scavenging activity towards the superoxide anion generated by the xanthine/xanthine oxidase assay system, as well as high Trolox Equivalent Antioxidant Capacity (TEAC), against the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)+?radical. TOFenriched extract exhibited the highest protective effect against free radicals, direct acting-mutagen and metabolically activated S9-dependent mutagens. Conclusions: The present study indicates that the extracts from A. salicina leaves are a significant source of compounds with the antimutagenic and antioxidant activities, and this may be useful for developing potential chemopreventive substances. Keywords: Acacia salicina, Antigenotoxic activity, Antioxidant activities, Ames assayBackground Plants are rich source of natural products used for centuries to cure various diseases. The plant-derived medicines are based upon the premise that they contain natural substances that can PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27597769 promote health and alleviate illness. So, a retrospection of the healing power of plants and a return to natural substances are an absolute need of our time. The demonstration of the presence of natural products, such as polyphenols, alkaloids, flavonoids, coumarins and* Correspondence: [email protected] Contributed equally 1 Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir 5000, Tunisia Full list of author information is available at the end of the articleother secondary metabolites in medicinal plants will provide a scientific validation for the popular use of these plants [1]. Acacia (Fabaceae) is an evergreen tree that is native of Australia, but it is now widely distributed in the Mediterranean area. Acacia is a large genus comprising more than 700 species. The genus Acacia is frequently used for the treatment of various illnesses because of their reputed pharmacological effects; published informations indicate that Acacia has hypoglycemic [2], antibacterial [3], antiinflammatory [4], cestocidal [5], spasmogenic and vasoconstrictor [6], antihypertensive and antispasmodic activities [7], anti-aggregation platelet effect [8], as well as an inhibitory effect against hepatitis C virus [9]. In Tunisian?2012 Boubaker et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Boubaker et al. BMC Complementary and Alternative Medicine 2012, 12:37 http://www.biomedcentral.com/1472-6882/12/Page 2 oftraditional medicine, the use of Acacia differs according to the species and according to the region. Based on informations gathered from tra.