With restricted antigen specificities can be conserved through development via CDWith restricted antigen specificities can

With restricted antigen specificities can be conserved through development via CD
With restricted antigen specificities can be conserved through development via CD5+ B cells, and during abnormal immune responses can potentially give rise to SLE-associated pathogenic autoantibodies.151 Enhanced proliferative response of CD4+ T cells PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28607003 from patients with systemic lupus erythematosus by T-cell receptor stimulationS Park1, I Kang2, H Kim1, J Craft2 of Rheumatology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea; 2Section of Rheumatology, Department of Internal Medicine, Yale University Medical School, New Haven, Quinagolide (hydrochloride) biological activity Connecticutt, USA Arthritis Res Ther 2003, 5(Suppl 3):151 (DOI 10.1186/ar952)1Division150 The systemic lupus erythematosus VJ repertoire harbors characteristics of the natural antibody repertoireJ Lee1, PE Lipsky2 of Internal Medicine, Ewha Womans University College of Medicine, Seoul, Korea; 2NIAMS, National Institutes of Health, Bethesda, Maryland, USA Arthritis Res Ther 2003, 5(Suppl 3):150 (DOI 10.1186/ar951)1DepartmentThe molecular mechanisms that lead to autoantibody production in systemic lupus erythematosus (SLE) are poorly understood. There is evidence that pathogenic IgG autoantibodies characteristic of human SLE may arise from the physiologically autoreactive natural antibody repertoire of fetal or CD5+ B cells. To address this hypothesis, the SLE V repertoire obtained from B cells of three SLE patients was analyzed and compared in detail with the V repertoire obtained from IgM+ B cells of three human fetal spleens and IgM+CD5+/CD5?B cells of two normal adults. VJ rearrangements were amplified from genomic DNA of individual B cells by PCR. The expressed V repertoire of SLE patients contained several similarities with the expressed repertoire of the fetal and adult CD5+ B cells. The V gene, 1G, was a major component of the SLE, fetal and adult CD5+ B-cell repertoire, but not in the adult CD5?B-cell population. The restriction of junctional diversity by utilization of homology-directed joining (H-joining) together with the absence of N-regions was a prominent feature of the fetal and adult CD5+ B-cell repertoires. This restriction was also observed in the SLE repertoire, but was less significant in the adult CD5?B-cell population. Furthermore, profound expansion of V clones employing identical CDR3s were observed in the adult CD5+ B cells, the fetal cells, and the SLE repertoire, whereas the frequency of the V clones were much lower in the adult CD5?B-cell population. Notably, significant PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26437915 numbers of expanded adult CD5+ B cells, and fetus and SLE V clones utilizedObjective To investigate the expression of the chemokine receptor CCR7, which defines distinct subsets of naive and memory T lymphocytes with different homing and effector capacities, and the proliferative capacity of CD4+ T cells by T-cell receptor (TCR) stimulation in patients with systemic lupus erythematosus (SLE) Methods Heparinized venous blood from SLE patients (23?3 years old; mean, 36.7 years) and age-matched and sex-matched healthy controls (HC) was collected. Peripheral blood mononuclear cells (PBMC) were freshly isolated by Ficoll-Hypaque. We stained 106 PBMC with anti-human CCR7 antibody, anti-CD45RA, anti-CD4 and anti-CD8 to characterize the phenotype of T-cell subsets. PBMC were stimulated with anti-CD3 + anti-CD28 monoclonal antibody, and cell division was analyzed by carboxyfluorescein diacetate succinimidyl ester labeling and flow cytometry in different subsets of CD4+ T cells. Data was acquired.