Recombinant Human TRMT12 Protein Summary
| Description |
TRMT12 (Human) GST-Tagged Recombinant Protein (P01)
Source: Wheat Germ (in vitro) Amino Acid Sequence: MRENVVVSNMERESGKPVAVVAVVTEPRFTQRYREYLQRQKLFDTQHRVEKMPDGSVALPVLGETLPEQHLQELRNRVAPGSPCMLTQLPDPVPSKRAQGCSPAQKLCLEVSRWVEGRGVKWSAELEADLPRSWQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVSPDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIADRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHWLYPQQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQPVKSYAPHVDHIVLDLECCPCPSVG |
| Preparation Method |
in vitro wheat germ expression system
|
| Details of Functionality |
This protein is not active and should not be used for experiments requiring activity.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
TRMT12
|
Applications/Dilutions
| Application Notes |
Useful in Western Blot and ELISA. This protein has not been tested for any functionality. This product may contain endotoxins and is not suitable for use with live cells.
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Recombinant Human TRMT12 Protein
- Alpha-amino-alpha-carboxypropyl transferase TYW2
- EC 2.1.1
- EC 2.1.1.32
- EC 2.5.1.-
- FLJ20772
- homolog of yeast tRNA methyltransferase
- TRM12
- tRNA methyltranferase 12 homolog (S. cerevisiae)
- tRNA methyltranferase 12 homolog
- tRNA methyltransferase 12 homolog (S. cerevisiae)
- tRNA-yW synthesizing protein 2
- tRNA-yW-synthesizing protein 2
- TYW2tRNA wybutosine-synthesizing protein 2 homolog
Background
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM]
