COLO 320 HSR Whole Cell Lysate Summary
| Description |
Whole cell lysate (non-denatured)
|
| Localization |
Colon
|
| Preparation Method |
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay.
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
In modified RIPA Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF).
|
| Concentration |
5 mg/ml
|
Lysate Details for COLO 320 HSR
| Type |
Cell
|
| Tissue |
Colon
|
| Protein State |
Denatured
|
| Subcellular Fraction |
Whole
|
Notes
Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.
This product is produced by and distributed for Abnova, a company based in Taiwan.
