Product Name :
[pSer15]HSP27 (human), ELISA kit
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Store all components at 4°C, except standard and antibody at -20°C.
Description:
Only commercially available ELISA kit for ultra-sensitive detection of human phospho-Ser15 HSP27 in cell lysates, plasma, and serum. Ultra-sensitive and specific – be confident with your results and use less sample per testQuantitative – obtain statistically significant results compared to semi-quantitative Western blot analysisHigher throughput – assay up to 40 samples in duplicate in just 3 hours The [pSer15]HSP27 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.{{129497-78-5} medchemexpress|{129497-78-5} Protocol|{129497-78-5} In Vitro|{129497-78-5} custom synthesis} Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.{{294-90-6} web|{294-90-6} Purity & Documentation|{294-90-6} Data Sheet|{294-90-6} custom synthesis} Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation.PMID:29939591 Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-α, p70 S6K, PKD I, and PKC-δ. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer. [pSer15]HSP27 (human), ELISA kit Standard curve [pSer15]HSP27 (human), ELISA kit Standard curve
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| Alternative Name Heat shock protein 27 | Application Colorimetric detection, ELISA | Application Notes For the quantitative determination of human [pSer15]HSP27 in cell lysates, plasma, and serum. | Assay Time 3 hours | Contents Microtiter plate, Conjugate, Antibody, Assay buffer 27, Wash buffer concentrate, Standard, TMB Substrate, 5X Extraction reagent, Stop solution 2 | Crossreactivity Phopho HSP27 (Ser15) (100%), HSP27 (non-phospho) (0.885%), HSP70 (0.023%) and | GenBank ID L39370 | Sensitivity 10.15 pg/ml (range 31.25 – 1000 pg/ml) | Species Reactivity Human | UniProt ID P04792 | Wavelength 450 nm
