The above conclusions are constant together with the analysis findings on Arabidopsis
The above conclusions are constant with all the investigation findings on Arabidopsis thaliana and rice. We also located that exogenous BR spraying upregulates theanine synthesis genes, namely, TS, GS, and ADC and cold resistance-related genes, namely, CBF, ICE. It could be inferred that exogenous BR spraying enhanced the theanine content in tea leaves and improved cold resistance of tea plants. Theanine (L-Theanine) is really a special absolutely free amino acid in tea and key component of tea. Our findings are concordant to the study benefits of Li et al. (2018). The effect of exogenous BR spraying on the growth and improvement of tea leaves and the signal transduction pathway of BR in tea leaves was revealed by transcriptome analysis. Our final results showed that the tea leaves sprayed with BRs had been significantly distinctive, plus the upregulated genes have been mainly connected to BR signal transduction, sucrose synthesis, chlorophyll synthesis, starch synthesis, flavonoid biosynthesis, cell division, theanine synthesis, and cold resistance. Moreover, we also identified that following spraying BR, the essential genes for caffeine synthesis had been down-regulated. Our study lays the foundation for elucidating the molecular mechanism of your BR signal transduction pathway in tea leaves and its regulatory part around the development and development of tea plants.Supplementary InformationThe on line version consists of supplementary material available at doi. org/10.1186/s12864-021-08179-9. Additional file 1. Acknowledgements We thank LetPub (www.letpub.com) for its linguistic help throughout the preparation of this manuscript. Authors’ contributions QJ designed and performed the experiments, analyzed the information, drafted the paper. ZW prepared figures and tables. YC, YL, and NT participated within the experiment and data analysis. SL, JH and ZL conceived with the analysis,Through KEGG enrichment and annotation, the UGP, SPS, GPI, PFP and EP genes involved in sucrose synthesis; the GSA, HEMD, POR, CHLH, and COA genes associated to ginseng chlorophyll synthesis; the AGPase,Jin et al. BMC Genomics(2022) 23:Web page 12 ofparticipated in coordination, information evaluation and interpretation, drafted and reviewed the manuscript. All the authors study and authorized the final manuscript. Funding This function was financially supported by the National Natural Science Foundation of China (32172629, U19A2030, 31670689), Provincial Organic Science Foundation of Hunan (2020JJ4358), and Specific Project of Central NOD-like Receptor (NLR) Compound Government Guiding Local Science and Technology Improvement (2019XF5041). Availability of data and supplies All of the information supporting our findings are contained inside the manuscript. All raw transcriptome information reported in this write-up happen to be deposited inside the Sequence Study Archive (SRA) beneath accession number PRJNA756445.9.ten. 11.12.DeclarationsEthics approval and consent to participate The tea trees used in this study came in the Chang’an Tea Teaching and Experimental Base of Hunan Anaplastic lymphoma kinase (ALK) Inhibitor drug Agricultural University, Changsha City, Hunan Province, China, and no material license was essential. Our study is in full compliance using the regulations from the system. This article has obtained a license to use KEGG application from Kanehisa Labs [402]. Consent for publication Not applicable. Competing interests The authors have no conflict of interest to declare. Author details 1 Crucial Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China. two National Investigation Center of Engineering and Technology for Utiliz.
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