Tients with diabetes. Solutions: Individuals at Concord Hospital with suspected CAD gave written informed consent

Tients with diabetes. Solutions: Individuals at Concord Hospital with suspected CAD gave written informed consent and were administered RIPC (sphygmomanometer around the arm, 3 5 min cycles, n = 31) or sham (n = 29) just before angiography, with recruitment ongoing. Blood was collected pre- and promptly post-RIPC/sham and plateletfree plasma generated. International coagulation/fibrinolytic possible was measured by overall haemostatic potential assay (Reddel et al. Siglec-2/CD22 Proteins Source Thromb Res. 2013; 131(five): 457462) and many fibrinolytic factors by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Analysis institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have potential as diagnostic and prognostic biomarkers. In atherosclerosis, the underlying lead to of heart attack and stroke, EV release can be dysregulated and their contents can mediate pro-inflammatory effects. Numerous markers have already been previously identified on uEV such as exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of these membrane bound carriers consist of microRNAs (miRs). miR-21 and miR-155 are crucial regulatory miRs which can be upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to have pro-atherogenic effects and miR-155 deficiency in murine models leads to lowered atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from patients diagnosed with coronary artery illness (CAD), classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (stable angina). uEVs from symptomatic and asymptomatic individuals had been isolated by means of benchtop centrifugation. The concentration and size of uEVs have been analysed via the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = ten per group). uEV surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Results: uEV concentration in symptomatic sufferers (median; 6.46E+9 particles/mL) was substantially decreased (p 0.05) when compared with asymptomatic patients (median; 1.25E+10 particles/mL). CD11B+ uEVs had been increased and CD16+ uEVs have been decreased inside the symptomatic sufferers (p 0.01). Moreover, the concentration of CD45+ EVs have been elevated in symptomatic individuals (p 0.001). Though uEV miR-21 was unchanged, miR-155 expression was significantly improved in the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic possible. As CAD severity increases, uEV concentration is lowered, surface marker expression is altered and uEV miR-155 expression is improved. Funding: The Irish Analysis Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal disease Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Division of CD100/Semaphorin-4D Proteins Biological Activity Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Health Evaluative Sciences, Investigation Institute, The Hospital for Sick Young children,.