Product: Hydroxyzine (D6 dihydrochloride)
SKOV-3 Nuclear Cell Lysate Summary
| Description |
Nuclear extract cell lysate (non-denatured)
|
| Localization |
Ovary
|
| Preparation Method |
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A and then centrifugated at 25000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged divided into aliquots and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2 mg/ml.
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
-2146826273
|
| Concentration |
2.5 mg/ml
|
Lysate Details for SKOV-3
| Type |
Cell
|
| Tissue |
Ovary
|
| Protein State |
Native
|
| Subcellular Fraction |
Nuclear
|
Notes
Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.
This product is produced by and distributed for Abnova, a company based in Taiwan.
