JAR Nuclear Cell Lysate | Potassium channel potassiun-channel.com

JAR Nuclear Cell Lysate Summary

Description	Nuclear extract cell lysate (non-denatured)
Localization	Placenta
Preparation Method	Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A and then centrifugated at 25000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged divided into aliquots and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2 mg/ml.

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	-2146826273
Concentration	2.5 mg/ml

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Description	Nuclear extract cell lysate (denatured)
Localization	Placenta
Preparation Method	Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A and then centrifugated at 25000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged divided into aliquots and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2.5 mg/ml and then mixed with 5X Sample Buffer to become final 2 mg/ml in Sample Buffer. The lysate was heated at 95C for 5 min and cooled rapidly.

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	-2146826273
Concentration	2 mg/ml

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.

This product is produced by and distributed for Abnova, a company based in Taiwan.